{"product_id":"new-product-401077","title":"Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit","description":"\u003cp\u003eHuman ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003eHuman ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eReactivity:\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003eACA-IgG; Anti-Cardiolipin Antibody IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type:\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity:\u003c\/td\u003e\n\u003ctd\u003e1.032 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard:\u003c\/td\u003e\n\u003ctd\u003e200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range:\u003c\/td\u003e\n\u003ctd\u003e3.13-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample Type:\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Length:\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area:\u003c\/td\u003e\n\u003ctd\u003eCardiovascular biology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle:\u003c\/td\u003e\n\u003ctd\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACA-IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACA-IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACA-IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACA-IgG in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item2\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eStandard curve\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"left fl\"\u003e\n\u003ctable class=\"table3\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration (ng\/mL)\u003c\/th\u003e\n\u003cth\u003eOD\u003c\/th\u003e\n\u003cth\u003eCorrected OD\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e200.00\u003c\/td\u003e\n\u003ctd\u003e1.929\u003c\/td\u003e\n\u003ctd class=\"1\"\u003e1.843\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100.00\u003c\/td\u003e\n\u003ctd\u003e1.543\u003c\/td\u003e\n\u003ctd class=\"2\"\u003e1.457\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50.00\u003c\/td\u003e\n\u003ctd\u003e1.115\u003c\/td\u003e\n\u003ctd class=\"3\"\u003e1.029\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e25.00\u003c\/td\u003e\n\u003ctd\u003e0.821\u003c\/td\u003e\n\u003ctd class=\"4\"\u003e0.735\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e12.50\u003c\/td\u003e\n\u003ctd\u003e0.557\u003c\/td\u003e\n\u003ctd class=\"5\"\u003e0.471\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6.25\u003c\/td\u003e\n\u003ctd\u003e0.319\u003c\/td\u003e\n\u003ctd class=\"6\"\u003e0.233\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3.13\u003c\/td\u003e\n\u003ctd\u003e0.206\u003c\/td\u003e\n\u003ctd class=\"7\"\u003e0.120\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.00\u003c\/td\u003e\n\u003ctd\u003e0.086\u003c\/td\u003e\n\u003ctd class=\"8\"\u003e0.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/ELISA\/ELK9253-b-1.jpg\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item3\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003ePrecision\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay)：\u003cspan class=\"point\"\u003eCV%\u0026lt;8%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested twenty times on one plate to assess intra-assay precision.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays)：\u003cspan class=\"point\"\u003eCV%\u0026lt;10%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested in forty separate assays to assess inter-assay precision.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eRecovery\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eMatrices listed below were spiked with certain level of recombinant ACA-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgG in samples.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth width=\"33.33%\"\u003eRecovery range\u003c\/th\u003e\n\u003cth\u003eAverage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e80-95%\u003c\/td\u003e\n\u003ctd\u003e87%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e87-101%\u003c\/td\u003e\n\u003ctd\u003e94%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e89-103%\u003c\/td\u003e\n\u003ctd\u003e96%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eLinearity\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth\u003e1:2\u003c\/th\u003e\n\u003cth\u003e1:4\u003c\/th\u003e\n\u003cth\u003e1:8\u003c\/th\u003e\n\u003cth\u003e1:16\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e93-101%\u003c\/td\u003e\n\u003ctd\u003e79-96%\u003c\/td\u003e\n\u003ctd\u003e92-105%\u003c\/td\u003e\n\u003ctd\u003e91-99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e90-98%\u003c\/td\u003e\n\u003ctd\u003e87-98%\u003c\/td\u003e\n\u003ctd\u003e92-104%\u003c\/td\u003e\n\u003ctd\u003e86-98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e81-98%\u003c\/td\u003e\n\u003ctd\u003e78-90%\u003c\/td\u003e\n\u003ctd\u003e91-99%\u003c\/td\u003e\n\u003ctd\u003e97-103%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":47453377560856,"sku":"ELK9253","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/ELK_8c6a8fbb-2f49-4e5a-ae38-89d21b7310a6.jpg?v=1704426188","url":"https:\/\/danabiosci.com\/products\/new-product-401077","provider":"Dana Bioscience","version":"1.0","type":"link"}