{"product_id":"new-product-397894","title":"Dog NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit","description":"\u003cp\u003eDog NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003eDog NOS2\/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eReactivity:\u003c\/td\u003e\n\u003ctd\u003eDog\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003eNOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type:\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity:\u003c\/td\u003e\n\u003ctd\u003e0.126 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard:\u003c\/td\u003e\n\u003ctd\u003e20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range:\u003c\/td\u003e\n\u003ctd\u003e0.32-20 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample Type:\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Length:\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area:\u003c\/td\u003e\n\u003ctd\u003eEnzyme \u0026amp; Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle:\u003c\/td\u003e\n\u003ctd\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog NOS2\/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NOS2\/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog NOS2\/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog NOS2\/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item2\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eStandard curve\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"left fl\"\u003e\n\u003ctable class=\"table3\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration (ng\/mL)\u003c\/th\u003e\n\u003cth\u003eOD\u003c\/th\u003e\n\u003cth\u003eCorrected OD\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20.00\u003c\/td\u003e\n\u003ctd\u003e2.354\u003c\/td\u003e\n\u003ctd class=\"1\"\u003e2.260\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10.00\u003c\/td\u003e\n\u003ctd\u003e1.582\u003c\/td\u003e\n\u003ctd class=\"2\"\u003e1.488\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5.00\u003c\/td\u003e\n\u003ctd\u003e1.193\u003c\/td\u003e\n\u003ctd class=\"3\"\u003e1.099\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2.50\u003c\/td\u003e\n\u003ctd\u003e0.941\u003c\/td\u003e\n\u003ctd class=\"4\"\u003e0.847\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1.25\u003c\/td\u003e\n\u003ctd\u003e0.547\u003c\/td\u003e\n\u003ctd class=\"5\"\u003e0.453\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.63\u003c\/td\u003e\n\u003ctd\u003e0.354\u003c\/td\u003e\n\u003ctd class=\"6\"\u003e0.260\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.32\u003c\/td\u003e\n\u003ctd\u003e0.194\u003c\/td\u003e\n\u003ctd class=\"7\"\u003e0.100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.00\u003c\/td\u003e\n\u003ctd\u003e0.094\u003c\/td\u003e\n\u003ctd class=\"8\"\u003e0.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/ELISA\/ELK6023-b-1.jpg\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item3\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003ePrecision\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay)：\u003cspan class=\"point\"\u003eCV%\u0026lt;8%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested twenty times on one plate to assess intra-assay precision.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays)：\u003cspan class=\"point\"\u003eCV%\u0026lt;10%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested in forty separate assays to assess inter-assay precision.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eRecovery\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eMatrices listed below were spiked with certain level of recombinant NOS2\/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2\/iNOS in samples.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth width=\"33.33%\"\u003eRecovery range\u003c\/th\u003e\n\u003cth\u003eAverage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e87-99%\u003c\/td\u003e\n\u003ctd\u003e93%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e93-107%\u003c\/td\u003e\n\u003ctd\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e85-99%\u003c\/td\u003e\n\u003ctd\u003e92%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eLinearity\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2\/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth\u003e1:2\u003c\/th\u003e\n\u003cth\u003e1:4\u003c\/th\u003e\n\u003cth\u003e1:8\u003c\/th\u003e\n\u003cth\u003e1:16\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e85-94%\u003c\/td\u003e\n\u003ctd\u003e87-103%\u003c\/td\u003e\n\u003ctd\u003e81-95%\u003c\/td\u003e\n\u003ctd\u003e82-90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e90-99%\u003c\/td\u003e\n\u003ctd\u003e95-106%\u003c\/td\u003e\n\u003ctd\u003e87-101%\u003c\/td\u003e\n\u003ctd\u003e82-97%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e90-99%\u003c\/td\u003e\n\u003ctd\u003e83-97%\u003c\/td\u003e\n\u003ctd\u003e81-103%\u003c\/td\u003e\n\u003ctd\u003e89-101%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96T \/ 0.126 ng\/mL \/ 0.32-20 ng\/mL","offer_id":47452344090904,"sku":"ELK6023","price":588.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/ELK_25f1d64e-0ea9-4bfb-8a6f-ff600587a69e.jpg?v=1704399777","url":"https:\/\/danabiosci.com\/products\/new-product-397894","provider":"Dana Bioscience","version":"1.0","type":"link"}