{"product_id":"new-product-394326","title":"Rat EDN1(Endothelin 1) ELISA Kit","description":"\u003cp\u003eRat EDN1(Endothelin 1) ELISA Kit\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\n\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003cspan class=\"fz20 fl tran300\"\u003ePublications\u003c\/span\u003e\n\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003eRat ET-1(Endothelin1) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eReactivity:\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003eET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type:\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity:\u003c\/td\u003e\n\u003ctd\u003e2.71 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard:\u003c\/td\u003e\n\u003ctd\u003e500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eRange:\u003c\/td\u003e\n\u003ctd\u003e7.82-500 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample Type:\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Length:\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area:\u003c\/td\u003e\n\u003ctd\u003eEndocrinology;Cardiovascular biology;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle:\u003c\/td\u003e\n\u003ctd\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"swiper-slide swiper-no-swiping swiper-slide-next\"\u003e\n\u003cdiv class=\"picList\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cdiv class=\"pic\"\u003e\n\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0022480419308066\" target=\"_blank\"\u003e\u003cem class=\"tran500 bgStyle\"\u003e\u003c\/em\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"text fz14\"\u003e\n\u003ch5 class=\"fz16 tran300\"\u003e\u003ca class=\"TXTovehid\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0022480419308066\" target=\"_blank\"\u003eJournal of Surgical Research\u003c\/a\u003e\u003c\/h5\u003e\n\u003cp class=\"\"\u003e\u003ca class=\"TXTovehid-two\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0022480419308066\" target=\"_blank\"\u003ePyruvate protects against intestinal injury by inhibiting the JAK\/STAT signaling pathway in rats with hemorrhagic shock\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"info clearfix\"\u003e\n\u003cspan class=\"fl\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/theme\/default\/images\/dateIcon1.svg\"\u003eDate：2021-10-20\u003c\/span\u003e\u003cspan class=\"fl\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/theme\/default\/images\/ifIcon1.svg\"\u003eIF：1.955\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item2\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eStandard curve\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"left fl\"\u003e\n\u003ctable class=\"table3\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration (ng\/mL)\u003c\/th\u003e\n\u003cth\u003eOD\u003c\/th\u003e\n\u003cth\u003eCorrected OD\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e500.00\u003c\/td\u003e\n\u003ctd\u003e2.045\u003c\/td\u003e\n\u003ctd class=\"1\"\u003e1.957\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e250.00\u003c\/td\u003e\n\u003ctd\u003e1.577\u003c\/td\u003e\n\u003ctd class=\"2\"\u003e1.489\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e125.00\u003c\/td\u003e\n\u003ctd\u003e1.069\u003c\/td\u003e\n\u003ctd class=\"3\"\u003e0.981\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e62.50\u003c\/td\u003e\n\u003ctd\u003e0.697\u003c\/td\u003e\n\u003ctd class=\"4\"\u003e0.609\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e31.25\u003c\/td\u003e\n\u003ctd\u003e0.593\u003c\/td\u003e\n\u003ctd class=\"5\"\u003e0.505\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e15.63\u003c\/td\u003e\n\u003ctd\u003e0.300\u003c\/td\u003e\n\u003ctd class=\"6\"\u003e0.212\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e7.82\u003c\/td\u003e\n\u003ctd\u003e0.216\u003c\/td\u003e\n\u003ctd class=\"7\"\u003e0.128\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.00\u003c\/td\u003e\n\u003ctd\u003e0.088\u003c\/td\u003e\n\u003ctd class=\"8\"\u003e0.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/ELISA\/ELK2436-b-1.jpg\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item3\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003ePrecision\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay)：\u003cspan class=\"point\"\u003eCV%\u0026lt;8%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested twenty times on one plate to assess intra-assay precision.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays)：\u003cspan class=\"point\"\u003eCV%\u0026lt;10%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested in forty separate assays to assess inter-assay precision.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eRecovery\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eMatrices listed below were spiked with certain level of recombinant ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth width=\"33.33%\"\u003eRecovery range\u003c\/th\u003e\n\u003cth\u003eAverage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e83-97%\u003c\/td\u003e\n\u003ctd\u003e90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e86-99%\u003c\/td\u003e\n\u003ctd\u003e92%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e89-103%\u003c\/td\u003e\n\u003ctd\u003e96%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eLinearity\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth\u003e1:2\u003c\/th\u003e\n\u003cth\u003e1:4\u003c\/th\u003e\n\u003cth\u003e1:8\u003c\/th\u003e\n\u003cth\u003e1:16\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e83-98%\u003c\/td\u003e\n\u003ctd\u003e88-97%\u003c\/td\u003e\n\u003ctd\u003e96-105%\u003c\/td\u003e\n\u003ctd\u003e86-92%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e89-97%\u003c\/td\u003e\n\u003ctd\u003e85-94%\u003c\/td\u003e\n\u003ctd\u003e79-96%\u003c\/td\u003e\n\u003ctd\u003e85-99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e79-93%\u003c\/td\u003e\n\u003ctd\u003e89-97%\u003c\/td\u003e\n\u003ctd\u003e85-94%\u003c\/td\u003e\n\u003ctd\u003e87-101%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96T \/ 2.71 pg\/mL \/ 7.82-500 pg\/mL","offer_id":47448426447128,"sku":"ELK2436","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/ELK_ac936db1-bb81-418e-9981-04550f1a7ed7.jpg?v=1704355909","url":"https:\/\/danabiosci.com\/products\/new-product-394326","provider":"Dana Bioscience","version":"1.0","type":"link"}