{"product_id":"new-product-393393","title":"Rat NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit","description":"\u003cp\u003eRat NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\n\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003cspan class=\"fz20 fl tran300\"\u003ePublications\u003c\/span\u003e\n\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003eRat NOS2\/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eReactivity:\u003c\/td\u003e\n\u003ctd\u003eRat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003eNOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type:\u003c\/td\u003e\n\u003ctd\u003eSandwich\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity:\u003c\/td\u003e\n\u003ctd\u003e0.62 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard:\u003c\/td\u003e\n\u003ctd\u003e100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range:\u003c\/td\u003e\n\u003ctd\u003e1.57-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample Type:\u003c\/td\u003e\n\u003ctd\u003etissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Length:\u003c\/td\u003e\n\u003ctd\u003e3.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area:\u003c\/td\u003e\n\u003ctd\u003eEnzyme \u0026amp; Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle:\u003c\/td\u003e\n\u003ctd\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2\/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2\/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2\/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2\/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"swiper-slide swiper-no-swiping swiper-slide-next\"\u003e\n\u003cdiv class=\"picList\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cdiv class=\"pic\"\u003e\n\u003ca href=\"https:\/\/linkspringer.53yu.com\/article\/10.1186\/s12974-021-02294-w#citeas\" target=\"_blank\"\u003e\u003cem class=\"tran500 bgStyle\"\u003e\u003c\/em\u003e\u003c\/a\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"text fz14\"\u003e\n\u003ch5 class=\"fz16 tran300\"\u003e\u003ca class=\"TXTovehid\" href=\"https:\/\/linkspringer.53yu.com\/article\/10.1186\/s12974-021-02294-w#citeas\" target=\"_blank\"\u003eJournal of Neuroinflammation\u003c\/a\u003e\u003c\/h5\u003e\n\u003cp class=\"\"\u003e\u003ca class=\"TXTovehid-two\" href=\"https:\/\/linkspringer.53yu.com\/article\/10.1186\/s12974-021-02294-w#citeas\" target=\"_blank\"\u003eRegulation of the p75 neurotrophin receptor attenuates neuroinflammation and stimulates hippocampal neurogenesis in experimental Streptococcus pneumoniae meningitis\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"info clearfix\"\u003e\n\u003cspan class=\"fl\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/theme\/default\/images\/dateIcon1.svg\"\u003eDate：2021-11-02\u003c\/span\u003e\u003cspan class=\"fl\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/theme\/default\/images\/ifIcon1.svg\"\u003eIF：8.322\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item2\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eStandard curve\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"left fl\"\u003e\n\u003ctable class=\"table3\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration (ng\/mL)\u003c\/th\u003e\n\u003cth\u003eOD\u003c\/th\u003e\n\u003cth\u003eCorrected OD\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100.00\u003c\/td\u003e\n\u003ctd\u003e1.979\u003c\/td\u003e\n\u003ctd class=\"1\"\u003e1.883\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50.00\u003c\/td\u003e\n\u003ctd\u003e1.562\u003c\/td\u003e\n\u003ctd class=\"2\"\u003e1.466\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e25.00\u003c\/td\u003e\n\u003ctd\u003e1.029\u003c\/td\u003e\n\u003ctd class=\"3\"\u003e0.933\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e12.50\u003c\/td\u003e\n\u003ctd\u003e0.885\u003c\/td\u003e\n\u003ctd class=\"4\"\u003e0.789\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6.25\u003c\/td\u003e\n\u003ctd\u003e0.561\u003c\/td\u003e\n\u003ctd class=\"5\"\u003e0.465\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3.13\u003c\/td\u003e\n\u003ctd\u003e0.357\u003c\/td\u003e\n\u003ctd class=\"6\"\u003e0.261\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1.57\u003c\/td\u003e\n\u003ctd\u003e0.268\u003c\/td\u003e\n\u003ctd class=\"7\"\u003e0.172\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.00\u003c\/td\u003e\n\u003ctd\u003e0.096\u003c\/td\u003e\n\u003ctd class=\"8\"\u003e0.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/ELISA\/ELK1503-b-1.jpg\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item3\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003ePrecision\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay)：\u003cspan class=\"point\"\u003eCV%\u0026lt;8%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested twenty times on one plate to assess intra-assay precision.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays)：\u003cspan class=\"point\"\u003eCV%\u0026lt;10%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested in forty separate assays to assess inter-assay precision.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eRecovery\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eMatrices listed below were spiked with certain level of recombinant NOS2\/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2\/iNOS in samples.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth width=\"33.33%\"\u003eRecovery range\u003c\/th\u003e\n\u003cth\u003eAverage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e81-97%\u003c\/td\u003e\n\u003ctd\u003e89%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e84-97%\u003c\/td\u003e\n\u003ctd\u003e90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e82-94%\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eLinearity\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2\/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth\u003e1:2\u003c\/th\u003e\n\u003cth\u003e1:4\u003c\/th\u003e\n\u003cth\u003e1:8\u003c\/th\u003e\n\u003cth\u003e1:16\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e88-104%\u003c\/td\u003e\n\u003ctd\u003e84-98%\u003c\/td\u003e\n\u003ctd\u003e95-105%\u003c\/td\u003e\n\u003ctd\u003e88-102%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e88-103%\u003c\/td\u003e\n\u003ctd\u003e83-94%\u003c\/td\u003e\n\u003ctd\u003e92-105%\u003c\/td\u003e\n\u003ctd\u003e89-97%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e87-102%\u003c\/td\u003e\n\u003ctd\u003e85-94%\u003c\/td\u003e\n\u003ctd\u003e87-101%\u003c\/td\u003e\n\u003ctd\u003e89-99%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96T \/ 0.62 ng\/mL \/ 1.57-100 ng\/mL","offer_id":47448241111320,"sku":"ELK1503","price":458.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/ELK_11d35f35-4e1b-49e2-bf1b-e98b77f529e9.jpg?v=1704352253","url":"https:\/\/danabiosci.com\/products\/new-product-393393","provider":"Dana Bioscience","version":"1.0","type":"link"}