{"product_id":"new-product-388821","title":"Cyclin E1 (phospho Thr77) Rabbit Polyclonal Antibody","description":"\u003cp\u003eCyclin E1 (phospho Thr77) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003eCyclin E1 (phospho Thr77) rabbit pAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003eCCNE1; CCNE; G1\/S-specific cyclin-E1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eApplications:\u003c\/td\u003e\n\u003ctd\u003eIHC;IF;ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eRecommended Dilutions:\u003c\/td\u003e\n\u003ctd\u003eImmunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/10000. Not yet tested in other applications.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eImmunogen:\u003c\/td\u003e\n\u003ctd\u003eThe antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr77. AA range:43-92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStorage:\u003c\/td\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStorage:\u003c\/td\u003e\n\u003ctd\u003e-20°C\/1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eClonality:\u003c\/td\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eIsotype:\u003c\/td\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eConcentration:\u003c\/td\u003e\n\u003ctd\u003e1 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eGeneID（Human）:\u003c\/td\u003e\n\u003ctd\u003e898\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eHuman Swiss-Prot No:\u003c\/td\u003e\n\u003ctd\u003eP24864\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eCellular localization:\u003c\/td\u003e\n\u003ctd\u003eNucleus .\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eBackground:\u003c\/td\u003e\n\u003ctd\u003eThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1\/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSpecies Reactivity:\u003c\/td\u003e\n\u003ctd\u003eHuman;Rat;Mouse;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item5\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca class=\"scaleimg\"\u003e\n\u003cdiv class=\"pic\"\u003e\u003cimg class=\"tran500\" alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/Antibodies\/pAb\/ES7919-c-1.jpg\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"text fz16\"\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cyclin E1 (Phospho-Thr77) Antibody\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca class=\"scaleimg\"\u003e\n\u003cdiv class=\"pic\"\u003e\u003cimg class=\"tran500\" alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/Antibodies\/pAb\/ES7919-c-2.jpg\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"text fz16\"\u003eImmunofluorescence analysis of HeLa cells, using Cyclin E1 (Phospho-Thr77) Antibody. The picture on the right is blocked with the phospho peptide.\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca class=\"scaleimg\"\u003e\n\u003cdiv class=\"pic\"\u003e\u003cimg class=\"tran500\" alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/Antibodies\/pAb\/ES7919-c-3.jpg\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"text fz16\"\u003eImmunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"100ul","offer_id":47447097180440,"sku":"ES7919","price":248.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/ELK_9f2c9fa8-f493-4480-a680-7ca28832665a.jpg?v=1704328861","url":"https:\/\/danabiosci.com\/products\/new-product-388821","provider":"Dana Bioscience","version":"1.0","type":"link"}