{"product_id":"new-product-387520","title":"Plk1 (phospho Ser137) Rabbit Polyclonal Antibody","description":"\u003cp\u003ePlk1 (phospho Ser137) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003ePlk1 (phospho Ser137) rabbit pAb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003ePLK1; PLK; Serine\/threonine-protein kinase PLK1; Polo-like kinase 1; PLK-1; Serine\/threonine-protein kinase 13; STPK13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eApplications:\u003c\/td\u003e\n\u003ctd\u003eWB;IF;ELISA;IHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eRecommended Dilutions:\u003c\/td\u003e\n\u003ctd\u003eWB 1:500-2000; IF\/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eImmunogen:\u003c\/td\u003e\n\u003ctd\u003eThe antiserum was produced against synthesized peptide derived from human PLK1 around the phosphorylation site of Ser137. AA range:103-152\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStorage:\u003c\/td\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStorage:\u003c\/td\u003e\n\u003ctd\u003e-20°C\/1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eClonality:\u003c\/td\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eIsotype:\u003c\/td\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eConcentration:\u003c\/td\u003e\n\u003ctd\u003e1 mg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eObserved Band:\u003c\/td\u003e\n\u003ctd\u003e68kD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eGeneID（Human）:\u003c\/td\u003e\n\u003ctd\u003e5347\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eHuman Swiss-Prot No:\u003c\/td\u003e\n\u003ctd\u003eP53350\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eCellular localization:\u003c\/td\u003e\n\u003ctd\u003eNucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . localization at the centrosome starts at the G1\/S transition (PubMed:24018379). During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A\/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle. Localization to the centrosome is required for S ph\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eBackground:\u003c\/td\u003e\n\u003ctd\u003eThe Ser\/Thr protein kinase encoded by this gene belongs to the CDC5\/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSpecies Reactivity:\u003c\/td\u003e\n\u003ctd\u003eHuman;Mouse;Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item5\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca class=\"scaleimg\"\u003e\n\u003cdiv class=\"pic\"\u003e\u003cimg class=\"tran500\" alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/Antibodies\/pAb\/ES6618-c-1.jpg\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"text fz16\"\u003eWestern blot analysis of PLK1 (Phospho-Ser137) Antibody. The lane on the right is blocked with the PLK1 (Phospho-Ser137) peptide.\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca class=\"scaleimg\"\u003e\n\u003cdiv class=\"pic\"\u003e\u003cimg class=\"tran500\" alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/Antibodies\/pAb\/ES6618-c-2.jpg\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"text fz16\"\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLK1 (Phospho-Ser137) Antibody\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca class=\"scaleimg\"\u003e\n\u003cdiv class=\"pic\"\u003e\u003cimg class=\"tran500\" alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/Antibodies\/pAb\/ES6618-c-3.jpg\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"text fz16\"\u003eImmunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"100ul","offer_id":47446890971416,"sku":"ES6618","price":248.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/ELK_bbdbcf3e-5f6f-465b-93f8-264aa292144f.jpg?v=1704323758","url":"https:\/\/danabiosci.com\/products\/new-product-387520","provider":"Dana Bioscience","version":"1.0","type":"link"}