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ELK Biotechnology
SKU:ES3357
Ribosomal Protein L12 Rabbit Polyclonal Antibody
Ribosomal Protein L12 Rabbit Polyclonal Antibody
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$248.00 USD
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Ribosomal Protein L12 Rabbit Polyclonal Antibody
Overview
| Product name: | Ribosomal Protein L12 rabbit pAb |
| Alternative Names: | RPL12; 60S ribosomal protein L12 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RPL12. AA range:61-110 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 18kD |
| GeneID(Human): | 6136 |
| Human Swiss-Prot No: | P30050 |
| Cellular localization: | nucleolus,cytosol,focal adhesion,membrane,cytosolic large ribosomal subunit,extracellular matrix,extracellular exosome, |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Ribosomal Protein L12 Polyclonal Antibody diluted at 1:2000 -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from HepG2 and HeLa cells, using RPL12 Antibody. The lane on the right is blocked with the synthesized peptide.
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