{"product_id":"new-product-329802","title":"Human B7-H3 Protein, mFc-His Tag","description":"\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003ePRODUCT DATA\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_target\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTARGET\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/www.dimabio.com\/target\/b7-h3\" rel=\"tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/target\/b7-h3\"\u003eB7-H3\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_synonyms\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eB7-H3;CD276;B7 homolog 3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_description\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDESCRIPTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecombinant human B7-H3 protein with C-terminal mouse Fc and 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_delivery\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIn Stock\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_uniprot-id\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUNIPROT ID\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eQ5ZPR3\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_expression-host\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eEXPRESSION HOST\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHEK293\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_tag\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTAG\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eC-Mouse Fc and 6×His Tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-characterization\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR CHARACTERIZATION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eB7-H3(Leu29-Pro245) mFc(Pro99-Lys330) 6×His\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-weight\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR WEIGHT\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe protein has a predicted molecular mass of 70-75 kDa after removal of the signal peptide.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_purity\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePURITY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_formulation-reconstitution\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eFORMULATION \u0026amp; RECONSTITUTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_storage-shipping\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSTORAGE \u0026amp; SHIPPING\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_background\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eBACKGROUND\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_usage\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUSAGE\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eResearch use only\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003eIMAGES\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-3417\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300-150x400.jpg\" alt=\"sp-PME100012 B7 H3 mFc His PAGE 300\" width=\"150\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300-150x400.jpg 150w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300-100x267.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300-64x171.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300-300x800.jpg 300w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300.jpg 354w\" sizes=\"(max-width: 150px) 100vw, 150px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100012-B7-H3-mFc-His-PAGE-300-150x400.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 1.\u003c\/strong\u003e Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2862\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-451x400.jpg\" alt=\"elisa-PME100012 ELISA Human B7 H3 mFc His\" width=\"451\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-451x400.jpg 451w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-100x89.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-64x57.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-901x800.jpg 901w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-768x682.jpg 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-510x453.jpg 510w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His.jpg 1173w\" sizes=\"(max-width: 451px) 100vw, 451px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-ELISA-Human-B7-H3-mFc-His-451x400.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 2. \u003c\/strong\u003eELISA plate pre-coated by 2 μg\/ml (100 μl\/well) Human B7-H3, mFc-His tagged protein (PME100012) can bind Anti-B7-H3 Neutralizing antibody (\u003ca href=\"https:\/\/www.dimabio.com\/product\/anti-b7-h3-enoblituzumab-biosimilar-mab\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/product\/anti-b7-h3-enoblituzumab-biosimilar-mab\" target=\"_blank\"\u003eBME100010\u003c\/a\u003e) in a linear range of 0.24-31.25 ng\/ml.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-11426\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-471x400.jpg\" alt=\"sp-PME100012 B7 H3 Fig.3 Elisa 2\" width=\"471\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-471x400.jpg 471w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-942x800.jpg 942w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-768x652.jpg 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-100x85.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-64x54.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2.jpg 1043w\" sizes=\"(max-width: 471px) 100vw, 471px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100012-B7-H3-Fig.3-Elisa-2-471x400.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFigure 3. ELISA plate pre-coated by 2 μg\/mL (100 μL\/well) Human B7-H3 Protein, mFc-His Tag (PME100012) can bind Anti-B7-H3 (TAA06 biosimilar) mAb (BME100181) in a linear range of 0.64–16 ng\/mL.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dima Biotech","offers":[{"title":"100ug \/ B7-H3 \/ Q5ZPR3","offer_id":47432274805016,"sku":"PME100012","price":478.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Dimabio_eeadbf6b-8a79-48ea-868f-dacae8b14cc5.png?v=1703986542","url":"https:\/\/danabiosci.com\/products\/new-product-329802","provider":"Dana Bioscience","version":"1.0","type":"link"}