{"product_id":"new-product-329773","title":"Human PD-1 Protein, hFc-His tag","description":"\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003ePRODUCT DATA\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_target\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTARGET\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/www.dimabio.com\/target\/pd-1\" rel=\"tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/target\/pd-1\"\u003ePD-1\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_synonyms\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePDCD1;PD1;CD279;SLEB2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_description\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDESCRIPTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecombinant human PD-1 protein with C-terminal human Fc and 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_delivery\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIn Stock\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_uniprot-id\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUNIPROT ID\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eQ15116\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_expression-host\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eEXPRESSION HOST\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHEK293\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_tag\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTAG\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eC-Human Fc and 6×His Tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-characterization\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR CHARACTERIZATION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePD-1(Leu25-Gln167) hFc(Glu99-Ala330) 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-weight\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR WEIGHT\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe protein has a predicted molecular mass of 42.9 kDa after removal of the signal peptide.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_purity\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePURITY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_formulation-reconstitution\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eFORMULATION \u0026amp; RECONSTITUTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_storage-shipping\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSTORAGE \u0026amp; SHIPPING\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_background\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eBACKGROUND\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB\/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_usage\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUSAGE\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eResearch use only\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003eIMAGES\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-3287\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP-132x400.jpg\" alt=\"sp-PME100462 PD1 hFc His SP\" width=\"132\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP-132x400.jpg 132w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP-100x303.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP-64x194.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP-264x800.jpg 264w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP.jpg 390w\" sizes=\"(max-width: 132px) 100vw, 132px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100462-PD1-hFc-His-SP-132x400.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 1. \u003c\/strong\u003eHuman PD-1 Protein, hFc-His Tag on SDS-PAGE under reducing condition.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2776\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-423x400.png\" alt=\"elisa-PME100462 PD1 hFc His ELISA Fig2\" width=\"423\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-423x400.png 423w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-100x95.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-64x61.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-846x800.png 846w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-768x726.png 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-1536x1452.png 1536w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-2048x1936.png 2048w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-510x482.png 510w\" sizes=\"(max-width: 423px) 100vw, 423px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100462-PD1-hFc-His-ELISA-Fig2-423x400.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 2.\u003c\/strong\u003e ELISA plate pre-coated by 2 μg\/ml (100 μl\/well) Human PD1, hFc-His tagged protein (PME100462) can bind Human PDL1, mFc-His tagged protein (\u003ca href=\"https:\/\/www.dimabio.com\/product\/human-pd-l1-protein-mfc-his-tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/product\/human-pd-l1-protein-mfc-his-tag\" target=\"_blank\"\u003ePME100023\u003c\/a\u003e) in a linear range of 62.5-251.1 ng\/ml.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2660\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3.png\" alt=\"fc-PME100462 PD1 hFc His FLOW Fig3\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3.png 300w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3-100x100.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3-64x64.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3-280x280.png 280w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3-247x247.png 247w\" sizes=\"(max-width: 300px) 100vw, 300px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig3.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 3. \u003c\/strong\u003eFlow cytometry analysis with  0.2 μg\/ml Human PD-1 Protein, hFc-His tag (PME100462) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2659\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig4-e1666576176132-381x400.png\" alt=\"fc-PME100462 PD1 hFc His FLOW Fig4\" width=\"272\" height=\"286\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig4-e1666576176132-381x400.png 381w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig4-e1666576176132-100x105.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig4-e1666576176132-64x67.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig4-e1666576176132.png 493w\" sizes=\"(max-width: 272px) 100vw, 272px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100462-PD1-hFc-His-FLOW-Fig4-e1666576176132-381x400.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 4.\u003c\/strong\u003e HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein(blue histogram)were surface stained with  2ug\/ml Human PD-1 Protein, hFc-His tag (PME100462)followed by PE-conjugated Goat anti-human IgG secondary antibody.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dima Biotech","offers":[{"title":"100ug \/ PD-1 \/ Q15116","offer_id":47432267006232,"sku":"PME100462","price":555.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Dimabio_5ee3bf1c-a006-4ef6-83d0-35f7f5449b2f.png?v=1703986423","url":"https:\/\/danabiosci.com\/products\/new-product-329773","provider":"Dana Bioscience","version":"1.0","type":"link"}