{"product_id":"new-product-329728","title":"Human CTLA-4 Protein, hFc tag","description":"\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003ePRODUCT DATA\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_target\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTARGET\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/www.dimabio.com\/target\/ctla-4\" rel=\"tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/target\/ctla-4\"\u003eCTLA-4\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_synonyms\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCTLA4;CD152\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_description\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDESCRIPTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecombinant human CTLA-4 protein with C-terminal human Fc tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_delivery\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIn Stock\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_uniprot-id\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUNIPROT ID\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eP16410\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_expression-host\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eEXPRESSION HOST\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHEK293\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_tag\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTAG\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eC-Human Fc Tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-characterization\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR CHARACTERIZATION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCTLA-4(Gln36-Ser161) hFc(Glu99-Ala330)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-weight\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR WEIGHT\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide.The apparent molecular mass of CTLA4-hFc is approximately 40-55 kDa due to glycosylation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_purity\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePURITY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_formulation-reconstitution\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eFORMULATION \u0026amp; RECONSTITUTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_storage-shipping\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSTORAGE \u0026amp; SHIPPING\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_background\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eBACKGROUND\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_usage\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUSAGE\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eResearch use only\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003eIMAGES\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-3272\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300-150x400.jpg\" alt=\"sp-PME100479 CTLA4 hFc PAGE 300\" width=\"150\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300-150x400.jpg 150w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300-100x267.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300-64x171.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300-300x800.jpg 300w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300.jpg 354w\" sizes=\"(max-width: 150px) 100vw, 150px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100479-CTLA4-hFc-PAGE-300-150x400.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 1.\u003c\/strong\u003e Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2764\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-416x400.png\" alt=\"elisa-PME100479 CTLA4 hFc ELISA Fig2\" width=\"416\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-416x400.png 416w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-100x96.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-64x62.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-832x800.png 832w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-768x738.png 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-1536x1476.png 1536w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-2048x1969.png 2048w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-510x490.png 510w\" sizes=\"(max-width: 416px) 100vw, 416px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100479-CTLA4-hFc-ELISA-Fig2-416x400.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 2. \u003c\/strong\u003eELISA plate pre-coated by 2 μg\/ml (100 μl\/well) Human CTLA-4, hFc tagged protein (PME100479) can bind Anti-CTLA4 Neutralizing antibody (\u003ca href=\"https:\/\/www.dimabio.com\/product\/anti-ctla4-ipilimumab-biosimilar-mab\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/product\/anti-ctla4-ipilimumab-biosimilar-mab\" target=\"_blank\"\u003eBME100022\u003c\/a\u003e) in a linear range of 0.64-80.0 ng\/ml.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2654\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FC-Fig3.jpg\" alt=\"fc-PME100479 CTLA4 hFc FC Fig3\" width=\"600\" height=\"300\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FC-Fig3.jpg 600w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FC-Fig3-100x50.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FC-Fig3-64x32.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FC-Fig3-510x255.jpg 510w\" sizes=\"(max-width: 600px) 100vw, 600px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FC-Fig3.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 3. \u003c\/strong\u003eHEK293 cell line transfected with irrelevant protein \u003cstrong data-mce-fragment=\"1\"\u003e(B)\u003c\/strong\u003e and human B7-2 \u003cstrong data-mce-fragment=\"1\"\u003e(A)\u003c\/strong\u003e were surface stained with Human CTLA4, hFc tagged protein (PME100479) 1μg\/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2653\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4.png\" alt=\"fc-PME100479 CTLA4 hFc FLOW Fig4\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4.png 300w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4-100x100.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4-64x64.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4-280x280.png 280w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4-247x247.png 247w\" sizes=\"(max-width: 300px) 100vw, 300px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100479-CTLA4-hFc-FLOW-Fig4.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 4.\u003c\/strong\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003eFlow cytometry analysis with 1ug\/ml Human CTLA4 Protein, hFc tag (PME100479) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dima Biotech","offers":[{"title":"100ug \/ CTLA-4 \/ P16410","offer_id":47432249934104,"sku":"PME100479","price":555.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Dimabio_5416bb98-5571-4a05-b83b-03c636db5175.png?v=1703986189","url":"https:\/\/danabiosci.com\/products\/new-product-329728","provider":"Dana Bioscience","version":"1.0","type":"link"}