{"product_id":"new-product-329477","title":"Human VEGFA Protein, His Tag","description":"\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003ePRODUCT DATA\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_target\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTARGET\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/www.dimabio.com\/target\/vegfa\" rel=\"tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/target\/vegfa\"\u003eVEGFA\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_synonyms\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eMVCD1;VEGF;VPF\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_description\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDESCRIPTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecombinant Human VEGFA Protein with C-terminal 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_delivery\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIn Stock\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_uniprot-id\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUNIPROT ID\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eP15692\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_expression-host\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eEXPRESSION HOST\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHEK293\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_tag\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTAG\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eC-6×His Tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-characterization\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR CHARACTERIZATION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eVEGFA(Ala27-Lys147) 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-weight\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR WEIGHT\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe protein has a predicted molecular mass of 14.8 kDa after removal of the signal peptide.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_purity\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePURITY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_formulation-reconstitution\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eFORMULATION \u0026amp; RECONSTITUTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_storage-shipping\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSTORAGE \u0026amp; SHIPPING\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_background\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eBACKGROUND\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis gene is a member of the PDGF\/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_usage\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUSAGE\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eResearch use only\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003eIMAGES\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-3330\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300-197x400.jpg\" alt=\"sp-PME100111 VEGFA His PAGE 300\" width=\"197\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300-197x400.jpg 197w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300-100x203.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300-64x130.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300-395x800.jpg 395w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300.jpg 437w\" sizes=\"(max-width: 197px) 100vw, 197px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100111-VEGFA-His-PAGE-300-197x400.jpg\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 1.\u003c\/strong\u003e Human VEGFA Protein, His Tag on SDS-PAGE under non-reducing(NR) and reducing(R) conditions.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2795\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-500x400.png\" alt=\"elisa-PME100111 VEGFA His ELISA Fig2\" width=\"500\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-500x400.png 500w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-100x80.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-64x51.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-1000x800.png 1000w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-768x614.png 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-1536x1229.png 1536w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-2048x1638.png 2048w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-510x408.png 510w\" sizes=\"(max-width: 500px) 100vw, 500px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100111-VEGFA-His-ELISA-Fig2-500x400.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 2. \u003c\/strong\u003eELISA plate pre-coated by 1 μg\/ml (100 μl\/well) Human VEGFA , His tagged protein (PME100111) can bind Anti-VEGFA Neutralizing antibody (\u003ca href=\"https:\/\/www.dimabio.com\/product\/anti-vegfa-bevacizumab-biosimiar-mab\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/product\/anti-vegfa-bevacizumab-biosimiar-mab\" target=\"_blank\"\u003eBME100061\u003c\/a\u003e) in a linear range of 3.2-16 ng\/ml.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2665\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100111-VEGFA-His-flow-VEGFR2-Fig3-368x400.png\" alt=\"fc-PME100111 VEGFA His flow转染VEGFR2 Fig3\" width=\"368\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100111-VEGFA-His-flow-VEGFR2-Fig3-368x400.png 368w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100111-VEGFA-His-flow-VEGFR2-Fig3-100x109.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100111-VEGFA-His-flow-VEGFR2-Fig3-64x70.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100111-VEGFA-His-flow-VEGFR2-Fig3.png 544w\" sizes=\"(max-width: 368px) 100vw, 368px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100111-VEGFA-His-flow-VEGFR2-Fig3-368x400.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 3. \u003c\/strong\u003e Flow cytometry analysis with 1μg\/mL Human VEGFA Protein, His tag (PME100111) on Expi293 cells transfected with human VEGFR2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dima Biotech","offers":[{"title":"100ug \/ VEGFA \/ P15692","offer_id":47432161624344,"sku":"PME100111","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Dimabio_528ff2a0-0302-4a39-89af-9f2ff6931f45.png?v=1703984967","url":"https:\/\/danabiosci.com\/products\/new-product-329477","provider":"Dana Bioscience","version":"1.0","type":"link"}