{"product_id":"new-product-329458","title":"Human CD117 Protein, His Tag","description":"\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003ePRODUCT DATA\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_target\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTARGET\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/www.dimabio.com\/target\/cd117\" rel=\"tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/target\/cd117\"\u003eCD117\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_synonyms\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSYNONYMS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eC-Kit;CD117;MASTC;PBT;SCFR;KIT\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_description\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDESCRIPTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecombinant human CD117 protein with C-terminal 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_delivery\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIn Stock\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_uniprot-id\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUNIPROT ID\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eP10721\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_expression-host\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eEXPRESSION HOST\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHEK293\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_tag\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eTAG\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eC-6×His Tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-characterization\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR CHARACTERIZATION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCD117(Gln26-Thr520) 6×His tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-weight\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eMOLECULAR WEIGHT\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe protein has a predicted molecular mass of 56.5 kDa after removal of the signal peptide.The apparent molecular mass of CD117-His is approximately 70-100 kDa due to glycosylation.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_purity\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003ePURITY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThe purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_formulation-reconstitution\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eFORMULATION \u0026amp; RECONSTITUTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eLyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_storage-shipping\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eSTORAGE \u0026amp; SHIPPING\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_background\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eBACKGROUND\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_usage\" data-mce-fragment=\"1\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\" data-mce-fragment=\"1\"\u003eUSAGE\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eResearch use only\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-section\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"row\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\" data-mce-fragment=\"1\"\u003e\n\u003ch5 class=\"uppercase mt\" data-mce-fragment=\"1\"\u003eIMAGES\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg decoding=\"async\" class=\"alignnone size-medium wp-image-3240\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300-150x400.jpg\" alt=\"sp-PME100519 CD117 His PAGE 300\" width=\"150\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300-150x400.jpg 150w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300-100x267.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300-64x171.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300-300x800.jpg 300w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300.jpg 354w\" sizes=\"(max-width: 150px) 100vw, 150px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/sp\/PME100519-CD117-His-PAGE-300-150x400.jpg\" fetchpriority=\"high\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 1. \u003c\/strong\u003eHuman CD117 Protein, His Tag on SDS-PAGE under reducing condition.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg decoding=\"async\" class=\"alignnone size-medium wp-image-2744\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-467x400.png\" alt=\"elisa-PME100519 CD117 His ELISA Fig2\" width=\"467\" height=\"400\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-467x400.png 467w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-100x86.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-64x55.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-935x800.png 935w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-768x657.png 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-1536x1314.png 1536w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-2048x1753.png 2048w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-510x436.png 510w\" sizes=\"(max-width: 467px) 100vw, 467px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/elisa\/PME100519-CD117-His-ELISA-Fig2-467x400.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 2.\u003c\/strong\u003e ELISA plate pre-coated by 1 μg\/ml (100 μl\/well) Human CD117, His tagged protein (PME100519) can bind Human SCF, hFc tagged protein (\u003ca href=\"https:\/\/www.dimabio.com\/product\/human-scf-protein-hfc-tag\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.dimabio.com\/product\/human-scf-protein-hfc-tag\" target=\"_blank\"\u003ePME100563\u003c\/a\u003e) in a linear range of 3.2-400 ng\/ml.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cimg loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100519-CD117-His-flowSCF-Fig3.png\" alt=\"fc-PME100519 CD117 His flowSCF Fig3\" width=\"399\" height=\"400\" class=\"alignnone size-full wp-image-7994\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100519-CD117-His-flowSCF-Fig3.png 399w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100519-CD117-His-flowSCF-Fig3-100x100.png 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100519-CD117-His-flowSCF-Fig3-64x64.png 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100519-CD117-His-flowSCF-Fig3-280x280.png 280w\" sizes=\"(max-width: 399px) 100vw, 399px\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/proteins\/fc\/PME100519-CD117-His-flowSCF-Fig3.png\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eFigure 3. \u003c\/strong\u003eFlow cytometry analysis with 15 μg\/mL Human CD117 Protein, His tag (PME100519) on Expi293 cells transfected with human SCF (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dima Biotech","offers":[{"title":"100ug \/ CD117 \/ P10721","offer_id":47432149664024,"sku":"PME100519","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Dimabio_91c1fa69-0253-400a-8c70-9700362cb32f.png?v=1703984869","url":"https:\/\/danabiosci.com\/products\/new-product-329458","provider":"Dana Bioscience","version":"1.0","type":"link"}