{"product_id":"new-product-326374","title":"Human ADORA2A full length protein-synthetic nanodisc","description":"\u003cstrong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/strong\u003e\n\u003cdiv class=\"product-section\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\"\u003e\n\u003ch5 class=\"uppercase mt\"\u003ePRODUCT DATA\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\"\u003e\n\u003ctable class=\"woocommerce-product-attributes shop_attributes\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_target\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eTARGET\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003e\u003ca rel=\"tag\" href=\"https:\/\/www.dimabio.com\/target\/adora2a\"\u003eADORA2A\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_synonyms\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eSYNONYMS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eA2aR; ADORA2; RDC8\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_description\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eDESCRIPTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eHuman ADORA2A full length protein-synthetic nanodisc\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_delivery\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eIn Stock\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_uniprot-id\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eUNIPROT ID\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eP29274\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_expression-host\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eEXPRESSION HOST\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eHEK293\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_protein-families\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003ePROTEIN FAMILIES\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eGPCR\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_protein-pathways\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003ePROTEIN PATHWAYS\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eCalcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_molecular-weight\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eMOLECULAR WEIGHT\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eThe human full length ADORA2A protein has a MW of 44.7 kDa\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_formulation-reconstitution\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eFORMULATION \u0026amp; RECONSTITUTION\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eLyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_storage-shipping\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eSTORAGE \u0026amp; SHIPPING\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eStore at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_background\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eBACKGROUND\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eA member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"woocommerce-product-attributes-item woocommerce-product-attributes-item--attribute_pa_usage\"\u003e\n\u003cth class=\"woocommerce-product-attributes-item__label\"\u003eUSAGE\u003c\/th\u003e\n\u003ctd class=\"woocommerce-product-attributes-item__value\"\u003e\n\u003cp\u003eResearch use only\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product-section\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"large-10 col pb-0 mb-0\"\u003e\n\u003cdiv class=\"large-8 col pb-0 mb-0\"\u003e\n\u003ch5 class=\"uppercase mt\"\u003eIMAGES\u003c\/h5\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel entry-content\"\u003e\n\u003cp\u003e\u003cimg loading=\"eager\" sizes=\"(max-width: 447px) 100vw, 447px\" data-lazy-loaded=\"1\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1-447x400.jpg 447w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1-100x90.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1-64x57.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1-893x800.jpg 893w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1-768x688.jpg 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1.jpg 1217w\" class=\"alignnone size-medium wp-image-8045 jetpack-lazy-image jetpack-lazy-image--handled\" height=\"400\" width=\"447\" alt=\"elisa-FLP100020 ADORA2A Fig.1 Elisa 1\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/elisa\/FLP100020-ADORA2A-Fig.1-Elisa-1-447x400.jpg\" decoding=\"async\" fetchpriority=\"high\"\u003e\u003cbr\u003eFigure1. Elisa plates were pre-coated with Flag Tag AODRA2A-Nanodisc (0.2μg\/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with ADORA2A-Nanodisc is 1.181ng\/ml.\u003cbr\u003e\u003cimg loading=\"eager\" sizes=\"(max-width: 400px) 100vw, 400px\" data-lazy-loaded=\"1\" srcset=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-400x400.jpg 400w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-100x100.jpg 100w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-64x64.jpg 64w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-800x800.jpg 800w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-280x280.jpg 280w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-768x768.jpg 768w, https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1.jpg 885w\" class=\"alignnone size-medium wp-image-8103 jetpack-lazy-image jetpack-lazy-image--handled\" height=\"400\" width=\"400\" alt=\"page-FLP100020 ADORA2A Fig.2 SDS PAGE 1\" src=\"https:\/\/www.dimabio.com\/wp-content\/uploads\/products\/flp\/page\/FLP100020-ADORA2A-Fig.2-SDS-PAGE-1-400x400.jpg\" decoding=\"async\"\u003e\u003cbr\u003eFigure2. Human ADORA2A-Nanodisc, Flag Tag on SDS-PAGE\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dima Biotech","offers":[{"title":"10μg","offer_id":47431087194392,"sku":"FLP100020","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Dimabio_5ea7c3ab-e473-43b4-9e5b-19e0ea82a2e4.png?v=1703965934","url":"https:\/\/danabiosci.com\/products\/new-product-326374","provider":"Dana Bioscience","version":"1.0","type":"link"}