{"product_id":"new-product-276738","title":"RAF1 (phospho-S289) polyclonal antibody","description":"\u003cp\u003eRaf-1 is an ubiquitously expressed cytoplasmic protein with intrinsic serine\/ threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"100ul \/ Rabbit \/ Human,Mouse","offer_id":45805955645720,"sku":"ABCBW04828","price":348.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/758-7580448_monoclonal-antibody-png-transparent-png_5dbbdf2e-92e2-4c27-8281-eef9e3bcc99a.png?v=1689743073","url":"https:\/\/danabiosci.com\/products\/new-product-276738","provider":"Dana Bioscience","version":"1.0","type":"link"}