{"product_id":"new-product-230386","title":"Tyrosinase monoclonal antibody","description":"\u003cp\u003eThe chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell . This assay can be used to either identify multiple proteins associated with a specific region of the genome or to identify the many regions of the genome bound by a particular protein . ChIP can be used to determine the specific order of recruitment of various proteins to a gene promoter or to \"measure\" the relative amount of a particular histone modification across an entire gene locus . In addition to histone proteins, the ChIP assay can be used to analyze binding of transcription factors and co-factors, DNA replication factors, and DNA repair proteins. When performing the ChIP assay, cells are first fixed with formaldehyde, a reversible protein-DNA cross-linking agent that \"preserves\" the protein-DNA interactions occurring in the cell . Cells are lysed and chromatin is harvested and fragmented using either sonication or enzymatic digestion. Fragmented chromatin is then immunoprecipitated with antibodies specific to a particular protein or histone modification. Any DNA sequences that are associated with the protein or histone modification of interest will co-precipitate as part of the cross-linked chromatin complex and the relative amount of that DNA sequence will be enriched by the immunoselection process. After immunoprecipitation, the protein-DNA cross-links are reversed and the DNA is purified. Standard PCR or quantitative real-time PCR are often used to measure the amount of enrichment of a particular DNA sequence by a protein-specific immunoprecipitation . Alternatively, the ChIP assay can be combined with genomic tiling micro-array (ChIP on chip) techniques, high throughput sequencing (ChIP-Seq), or cloning strategies, all of which allow for genome-wide analysis of protein-DNA interactions and histone modifications . SimpleChIP® primers have been optimized for amplification of ChIP-isolated DNA using real-time quantitative PCR and provide important positive and negative controls that can be used to confirm a successful ChIP experiment.\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"100ul \/ Mouse \/ Human","offer_id":47662874427672,"sku":"MB66145","price":325.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/BIOWORLD_45a383f7-e07d-4752-bc92-38f8a418eb9a.jpg?v=1707498155","url":"https:\/\/danabiosci.com\/products\/new-product-230386","provider":"Dana Bioscience","version":"1.0","type":"link"}