{"product_id":"new-product-220518","title":"Human Kynurenine (KYN)ELISA Kit","description":"\u003cp\u003e\u003cstrong\u003eFor the quantitative determination of endogenic human kynurenine (KYN)\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003econcentrations in serum, urine, tissue homogenates.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePRINCIPLE OF THE ASSAY\u003c\/strong\u003e\u003cbr\u003eThis assay employs the competitive inhibition enzyme immunoassay technique.\u003cbr\u003eThe microtiter plate provided in this kit has been pre-coated with an antigen.\u003cbr\u003eStandards or samples are added to the appropriate microtiter plate wells with\u003cbr\u003eantibody specific for KYN and Horseradish Peroxidase (HRP) conjugated\u003cbr\u003egoat-anti-rabbit antibody. The competitive inhibition reaction is launched\u003cbr\u003ebetween with pre-coated KYN and KYN in samples. A substrate solution is\u003cbr\u003eadded to the wells and the color develops in opposite to the amount of KYN in\u003cbr\u003ethe sample. The color development is stopped and the intensity of the color is\u003cbr\u003emeasured.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eDETECTION RANGE\u003c\/strong\u003e\u003cbr\u003e7.8 pmol\/ml-500 pmol\/ml.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eSENSITIVITY\u003c\/strong\u003e\u003cbr\u003eThe minimum detectable dose of human KYN is typically less than 3.9 pmol\/ml.\u003cbr\u003eThe sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as\u003cbr\u003ethe lowest human KYN concentration that could be differentiated from zero.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eSPECIFICITY\u003c\/strong\u003e\u003cbr\u003eThis assay has high sensitivity and excellent specificity for detection of human\u003cbr\u003eKYN. No significant cross-reactivity or interference between human KYN and\u003cbr\u003eanalogues was observed.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eNote\u003c\/strong\u003e: Limited by current skills and knowledge, it is impossible for us to complete\u003cbr\u003ethe cross-reactivity detection between human KYN and all the analogues,\u003cbr\u003etherefore, cross reaction may still exist.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePRECISION\u003c\/strong\u003e\u003cbr\u003eIntra-assay Precision (Precision within an assay): CV%\u0026lt;8%\u003cbr\u003eThree samples of known concentration were tested twenty times on one plate to\u003cbr\u003eassess.\u003cbr\u003eInter-assay Precision (Precision between assays): CV%\u0026lt;10%\u003cbr\u003eThree samples of known concentration were tested in twenty assays to assess.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eLIMITATIONS OF THE PROCEDURE\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTICPROCEDURES.\u003c\/li\u003e\n\u003cli\u003eThe kit should not be used beyond the expiration date on the kit label.\u003c\/li\u003e\n\u003cli\u003eDo not mix or substitute reagents with those from other lots or sources.\u003c\/li\u003e\n\u003cli\u003eIf samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.\u003c\/li\u003e\n\u003cli\u003eAny variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.\u003c\/li\u003e\n\u003cli\u003eThis assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cusabio","offers":[{"title":"96T \/ 3.9 pmol\/ml \/ 7.8 pmol\/ml-500 pmol\/ml","offer_id":47660189384984,"sku":"CSB-E13659h","price":790.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/Cusabio_logo_d3a0b849-ec48-45fc-af68-5116a5ecb297.jpg?v=1707429283","url":"https:\/\/danabiosci.com\/products\/new-product-220518","provider":"Dana Bioscience","version":"1.0","type":"link"}