{"product_id":"new-product-135634","title":"Human EGF(Epidermal Growth Factor) ELISA Kit","description":"\u003cp\u003eThe Human EGF (Epidermal Growth Factor) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative measurement of human EGF in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of the target protein.\u003c\/p\u003e\n\u003cp\u003eThe assay is performed in a 96-well microplate coated with a monoclonal antibody against human EGF. The sample is added to the wells, and the EGF present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to human EGF is added to the wells, which binds to the captured EGF. Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured using a microplate reader. The concentration of human EGF in the sample is determined by comparing the optical density of the sample to a standard curve. This kit is highly sensitive, with a detection range of 7.8-500 pg\/mL, and has a high degree of reproducibility and accuracy. It is an essential tool for researchers and clinicians studying the role of EGF in various physiological and pathological processes.\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"96T","offer_id":47626861969688,"sku":"E-EL-H0059","price":495.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/elisa-1_8698a027-b96d-4808-9b9e-2f16da5d1aea.jpg?v=1706837872","url":"https:\/\/danabiosci.com\/products\/new-product-135634","provider":"Dana Bioscience","version":"1.0","type":"link"}