{"product_id":"new-product-134712","title":"Human TSP-1(Thrombospondin-1) ELISA Kit","description":"The Human TSP-1 (Thrombospondin-1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of TSP-1 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of TSP-1. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample and the detection antibody to the wells, followed by incubation and washing steps. The HRP substrate is then added, and the reaction is stopped by the addition of an acidic stop solution. The absorbance is measured at 450 nm, and the concentration of TSP-1 in the sample is determined by comparing the absorbance to a standard curve.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003eThe Human TSP-1 ELISA Kit has a detection range of 0.156-10 ng\/mL, with a sensitivity of 0.078 ng\/mL. The kit has been validated for use with human serum, plasma, and cell culture supernatants, and has been shown to have excellent intra- and inter-assay precision and accuracy. The kit is easy to use, with a total assay time of approximately 3 hours, and requires only a small sample volume (50 µL). The kit contains all the necessary reagents and components, including the microplate, the capture and detection antibodies, the HRP conjugate, the substrate, and the stop solution.\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003eThe Human TSP-1 ELISA Kit is a valuable tool for researchers studying the role of TSP-1 in various biological processes, including angiogenesis, wound healing, and cancer progression. The kit provides a reliable and accurate method for quantifying TSP-1 levels in human samples, and can be used to monitor changes in TSP-1 expression under different experimental conditions. The kit is also suitable for use in clinical settings, where it may be used to diagnose and monitor diseases associated with altered TSP-1 levels, such as cardiovascular disease and cancer.","brand":"Elabscience","offers":[{"title":"96T","offer_id":47626711597336,"sku":"E-EL-H1589","price":609.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/elisa-1_8c756f8d-30fd-4b22-a75d-38641223f329.jpg?v=1706833726","url":"https:\/\/danabiosci.com\/products\/new-product-134712","provider":"Dana Bioscience","version":"1.0","type":"link"}