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SKU:E-UNEL-H0135
Uncoated Human IP-10/CXCL10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
Uncoated Human IP-10/CXCL10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
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The Uncoated Human IP-10/CXCL10 ELISA Kit is a highly reliable and efficient tool designed for the quantitative measurement of Interferon Gamma Induced Protein 10kDa (IP-10/CXCL10) in human samples. This ELISA kit is specifically developed to provide accurate and precise results, making it an essential asset for researchers and scientists working in the field of immunology and cytokine research.
This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high sensitivity and specificity. The assay is performed in a 96-well plate coated with a capture antibody that specifically binds to IP-10/CXCL10 present in the sample. After the initial incubation, the unbound substances are washed away, and a biotin-conjugated detection antibody is added, forming a sandwich complex with the captured IP-10/CXCL10. This complex is then detected using streptavidin-horseradish peroxidase (HRP) conjugate and a chromogenic substrate. The intensity of the color developed is directly proportional to the concentration of IP-10/CXCL10 in the sample, which can be measured spectrophotometrically at a wavelength of 450 nm.
The Uncoated Human IP-10/CXCL10 ELISA Kit offers several advantages, including its wide dynamic range, high sensitivity, and excellent reproducibility. With a detection range of 7.8-500 pg/mL, this kit allows for the accurate quantification of IP-10/CXCL10 even at low concentrations. Furthermore, the kit exhibits a low limit of detection (LOD) of less than 2 pg/mL, ensuring the detection of even trace amounts of IP-10/CXCL10. The assay also demonstrates excellent intra-assay and inter-assay precision, with coefficients of variation (CV) less than 10% and 12%, respectively. This ELISA kit provides a user-friendly protocol, with a total assay time of approximately 3 hours, enabling efficient and time-saving experimentation. Overall, the Uncoated Human IP-10/CXCL10 ELISA Kit is a valuable tool for researchers seeking accurate and reliable quantification of IP-10/CXCL10 in human samples, facilitating advancements in immunological and cytokine-related studies.
This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high sensitivity and specificity. The assay is performed in a 96-well plate coated with a capture antibody that specifically binds to IP-10/CXCL10 present in the sample. After the initial incubation, the unbound substances are washed away, and a biotin-conjugated detection antibody is added, forming a sandwich complex with the captured IP-10/CXCL10. This complex is then detected using streptavidin-horseradish peroxidase (HRP) conjugate and a chromogenic substrate. The intensity of the color developed is directly proportional to the concentration of IP-10/CXCL10 in the sample, which can be measured spectrophotometrically at a wavelength of 450 nm.
The Uncoated Human IP-10/CXCL10 ELISA Kit offers several advantages, including its wide dynamic range, high sensitivity, and excellent reproducibility. With a detection range of 7.8-500 pg/mL, this kit allows for the accurate quantification of IP-10/CXCL10 even at low concentrations. Furthermore, the kit exhibits a low limit of detection (LOD) of less than 2 pg/mL, ensuring the detection of even trace amounts of IP-10/CXCL10. The assay also demonstrates excellent intra-assay and inter-assay precision, with coefficients of variation (CV) less than 10% and 12%, respectively. This ELISA kit provides a user-friendly protocol, with a total assay time of approximately 3 hours, enabling efficient and time-saving experimentation. Overall, the Uncoated Human IP-10/CXCL10 ELISA Kit is a valuable tool for researchers seeking accurate and reliable quantification of IP-10/CXCL10 in human samples, facilitating advancements in immunological and cytokine-related studies.
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