{"product_id":"mini-samples-elisa-kit-for-nesfatin-1-nes1-abcc22740","title":"Mini Samples ELISA Kit for Nesfatin 1 (NES1)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e493.8-40,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eMus musculus (Mouse)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 187.7pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Nesfatin 1 (NES1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Nesfatin 1 (NES1) and unlabeled Mini Samples Nesfatin 1 (NES1) (Standards or samples) with the pre-coated antibody specific to Mini Samples Nesfatin 1 (NES1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Nesfatin 1 (NES1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Nesfatin 1 (NES1) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eNesfatin 1\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 187.7pg\/mL \/ Mus musculus (Mouse)","offer_id":42481464213738,"sku":"ABCC22740","price":778.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_9024cf60-d7d9-4653-821b-4502dff80905.png?v=1644862378","url":"https:\/\/danabiosci.com\/products\/mini-samples-elisa-kit-for-nesfatin-1-nes1-abcc22740","provider":"Dana Bioscience","version":"1.0","type":"link"}