{"product_id":"gst-monoclonal-antibody-abccs01290","title":"GST Monoclonal Antibody","description":"\u003ch4\u003eSize\u003c\/h4\u003e\u003cp\u003e100ug\u003c\/p\u003e\u003ch4\u003eClone Number\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eAliases\u003c\/h4\u003e\u003cp\u003eGlutathione S-Transferase, gst, GST tag\u003c\/p\u003e\u003ch4\u003eImmunogen Species\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eUniProt ID\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eImmunogen\u003c\/h4\u003e\u003cp\u003eRecombinant GST Protein\u003c\/p\u003e\u003ch4\u003eRaised in\u003c\/h4\u003e\u003cp\u003eMouse\u003c\/p\u003e\u003ch4\u003eSpecies Reactivity\u003c\/h4\u003e\u003cp\u003eAll\u003c\/p\u003e\u003ch4\u003eTested Applications\u003c\/h4\u003e\u003cp\u003eELISA, WB; Recommended dilution:WB:1:500-1:5000\u003c\/p\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cp\u003eGenetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.\u003c\/p\u003e\u003ch4\u003eClonality\u003c\/h4\u003e\u003cp\u003eMonoclonal\u003c\/p\u003e\u003ch4\u003eIsotype\u003c\/h4\u003e\u003cp\u003eIgG2a\u003c\/p\u003e\u003ch4\u003ePurification Method\u003c\/h4\u003e\u003cp\u003e\u0026gt;95%,Protein G purified\u003c\/p\u003e\u003ch4\u003eConjugate\u003c\/h4\u003e\u003cp\u003eNon-conjugated\u003c\/p\u003e\u003ch4\u003eBuffer\u003c\/h4\u003e\u003cp\u003ePreservative: 0.03% Proclin 300\u003cbr\u003eConstituents: 50% Glycerol, 0.01M PBS, PH 7.4\u003c\/p\u003e\u003ch4\u003eForm\u003c\/h4\u003e\u003cp\u003eLiquid\u003c\/p\u003e\u003ch4\u003eStroage\u003c\/h4\u003e\u003cp\u003eUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003ch4\u003eTarget Names\u003c\/h4\u003e\u003cp\u003eGST\u003c\/p\u003e\u003ch4\u003eResearch Areas\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"Default Title","offer_id":42971187183850,"sku":"ABCCS01290","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/758-7580448_monoclonal-antibody-png-transparent-png_7d85ea21-f99b-498b-822c-41d50b54d591.png?v=1653294941","url":"https:\/\/danabiosci.com\/products\/gst-monoclonal-antibody-abccs01290","provider":"Dana Bioscience","version":"1.0","type":"link"}