{"product_id":"gapdh-monoclonal-antibody-abccs01301","title":"GAPDH Monoclonal Antibody","description":"\u003ch4\u003eSize\u003c\/h4\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003ch4\u003eClone Number\u003c\/h4\u003e\u003cp\u003e14C2F11\u003c\/p\u003e\u003ch4\u003eAliases\u003c\/h4\u003e\u003cp\u003eGAPDH; G3PD; GAPD; MGC88685\u003c\/p\u003e\u003ch4\u003eImmunogen Species\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eUniProt ID\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eImmunogen\u003c\/h4\u003e\u003cp\u003eRecombinant Human GAPDH protein (3-335AA)\u003c\/p\u003e\u003ch4\u003eRaised in\u003c\/h4\u003e\u003cp\u003eMouse\u003c\/p\u003e\u003ch4\u003eSpecies Reactivity\u003c\/h4\u003e\u003cp\u003eHuman, Rat, Rabbit\u003c\/p\u003e\u003ch4\u003eTested Applications\u003c\/h4\u003e\u003cp\u003eELISA, WB, IHC, IP, IF, FC; Recommended dilution: WB:1:5000-1:1600000, IHC:1:50-1:500, IF:1:50-1:200, IP:1µl-2µl, FC:1:100-1:300\u003c\/p\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cp\u003eGlyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and\/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1’s nuclear localization signal, and degrades Siah1’s substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer’s disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson’s disease, binds to GAPDH and displays neuroprotective actions.\u003c\/p\u003e\u003ch4\u003eClonality\u003c\/h4\u003e\u003cp\u003eMonoclonal\u003c\/p\u003e\u003ch4\u003eIsotype\u003c\/h4\u003e\u003cp\u003eIgG1\u003c\/p\u003e\u003ch4\u003ePurification Method\u003c\/h4\u003e\u003cp\u003e\u0026gt;95%, Protein G purified\u003c\/p\u003e\u003ch4\u003eConjugate\u003c\/h4\u003e\u003cp\u003eNon-conjugated\u003c\/p\u003e\u003ch4\u003eBuffer\u003c\/h4\u003e\u003cp\u003ePreservative: 0.03% Proclin 300\u003cbr\u003eConstituents: 50% Glycerol, 0.01M PBS, PH 7.4\u003c\/p\u003e\u003ch4\u003eForm\u003c\/h4\u003e\u003cp\u003eLiquid\u003c\/p\u003e\u003ch4\u003eStroage\u003c\/h4\u003e\u003cp\u003eUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003ch4\u003eTarget Names\u003c\/h4\u003e\u003cp\u003eGAPDH\u003c\/p\u003e\u003ch4\u003eResearch Areas\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"Default Title","offer_id":42971188265194,"sku":"ABCCS01301","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/758-7580448_monoclonal-antibody-png-transparent-png_fbefb30a-25db-40a8-83b2-c1595c609e8a.png?v=1653294959","url":"https:\/\/danabiosci.com\/products\/gapdh-monoclonal-antibody-abccs01301","provider":"Dana Bioscience","version":"1.0","type":"link"}