{"product_id":"flag-tag-monoclonal-antibody-abccs01299","title":"Flag Tag Monoclonal Antibody","description":"\u003ch4\u003eSize\u003c\/h4\u003e\u003cp\u003e100ul\u003c\/p\u003e\u003ch4\u003eClone Number\u003c\/h4\u003e\u003cp\u003e4G5C8\u003c\/p\u003e\u003ch4\u003eAliases\u003c\/h4\u003e\u003cp\u003eDDK tag, DYKDDDDK, Flag, Flag Tag\u003c\/p\u003e\u003ch4\u003eImmunogen Species\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eUniProt ID\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eImmunogen\u003c\/h4\u003e\u003cp\u003eDYKDDDDK(Flag) synthetic peptide conjugate to KLH\u003c\/p\u003e\u003ch4\u003eRaised in\u003c\/h4\u003e\u003cp\u003eMouse\u003c\/p\u003e\u003ch4\u003eSpecies Reactivity\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eTested Applications\u003c\/h4\u003e\u003cp\u003eELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl\u003c\/p\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cp\u003eFlag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.\u003c\/p\u003e\u003ch4\u003eClonality\u003c\/h4\u003e\u003cp\u003eMonoclonal\u003c\/p\u003e\u003ch4\u003eIsotype\u003c\/h4\u003e\u003cp\u003eIgG2a\u003c\/p\u003e\u003ch4\u003ePurification Method\u003c\/h4\u003e\u003cp\u003e\u0026gt;95%, Protein G purified\u003c\/p\u003e\u003ch4\u003eConjugate\u003c\/h4\u003e\u003cp\u003eNon-conjugated\u003c\/p\u003e\u003ch4\u003eBuffer\u003c\/h4\u003e\u003cp\u003ePreservative: 0.03% Proclin 300\u003cbr\u003eConstituents: 50% Glycerol, 0.01M PBS, PH 7.4\u003c\/p\u003e\u003ch4\u003eForm\u003c\/h4\u003e\u003cp\u003eLiquid\u003c\/p\u003e\u003ch4\u003eStroage\u003c\/h4\u003e\u003cp\u003eUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003ch4\u003eTarget Names\u003c\/h4\u003e\u003cp\u003eFlag Tag\u003c\/p\u003e\u003ch4\u003eResearch Areas\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"Default Title","offer_id":42971188134122,"sku":"ABCCS01299","price":200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/758-7580448_monoclonal-antibody-png-transparent-png_e62398df-750e-4752-856d-39a6b7bf4285.png?v=1653294955","url":"https:\/\/danabiosci.com\/products\/flag-tag-monoclonal-antibody-abccs01299","provider":"Dana Bioscience","version":"1.0","type":"link"}