{"product_id":"es3423","title":"Separase rabbit pAb","description":"\u003cstrong\u003eApplications:\u003c\/strong\u003e WB;IHC;IF;ELISA\u003cbr\u003e\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human;Mouse\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Rabbit\u003cbr\u003e\u003cstrong\u003eDilution:\u003c\/strong\u003e Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/40000. Not yet tested in other applications.\u003cbr\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816\u003cbr\u003e\u003cstrong\u003eStorage_stability:\u003c\/strong\u003e -20°C\/1 year\u003cbr\u003e\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal\u003cbr\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG\u003cbr\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1 mg\/ml\u003cbr\u003e\u003cstrong\u003eObserved_band(KD):\u003c\/strong\u003e 230kD\u003cbr\u003e\u003cstrong\u003eHuman_gene_id:\u003c\/strong\u003e 9700\u003cbr\u003e\u003cstrong\u003eHuman_swiss_prot_no:\u003c\/strong\u003e Q14674\u003cbr\u003e\u003cstrong\u003eSubcellular_location:\u003c\/strong\u003e Cytoplasm. Nucleus.\u003cbr\u003e\u003cstrong\u003eOther_name:\u003c\/strong\u003e ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010],","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":50411272372504,"sku":"ES3423","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ES3423-c-1.jpg?v=1751077765","url":"https:\/\/danabiosci.com\/products\/es3423","provider":"Dana Bioscience","version":"1.0","type":"link"}