{"product_id":"es3194","title":"PHLDA3 rabbit pAb","description":"\u003cstrong\u003eApplications:\u003c\/strong\u003e WB;IHC;IF;ELISA\u003cbr\u003e\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human;Mouse;Rat\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Rabbit\u003cbr\u003e\u003cstrong\u003eDilution:\u003c\/strong\u003e Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/40000. Not yet tested in other applications.\u003cbr\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e The antiserum was produced against synthesized peptide derived from human PHLDA3. AA range:21-70\u003cbr\u003e\u003cstrong\u003eStorage_stability:\u003c\/strong\u003e -20°C\/1 year\u003cbr\u003e\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal\u003cbr\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG\u003cbr\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1 mg\/ml\u003cbr\u003e\u003cstrong\u003eObserved_band(KD):\u003c\/strong\u003e 15kD\u003cbr\u003e\u003cstrong\u003eHuman_gene_id:\u003c\/strong\u003e 23612\u003cbr\u003e\u003cstrong\u003eHuman_swiss_prot_no:\u003c\/strong\u003e Q9Y5J5\u003cbr\u003e\u003cstrong\u003eSubcellular_location:\u003c\/strong\u003e Cytoplasm . Membrane ; Peripheral membrane protein .\u003cbr\u003e\u003cstrong\u003eOther_name:\u003c\/strong\u003e PHLDA3; TIH1; Pleckstrin homology-like domain family A member 3; TDAG51\/Ipl homolog 1\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e domain:The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.,function:p53\/TP53-regulated repressor of Akt\/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53\/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53\/TP53 may explain how p53\/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.,induction:By p53\/TP53; expression is directly activated by p53\/TP53. p53\/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter.,miscellaneous:PHLDA3 genomic locus is frequently observed in primary lung cancers, suggesting a role in tumor suppression.,similarity:Belongs to the PHLDA3 family.,similarity:Contains 1 PH domain.,tissue specificity:Widely expressed with lowest expression in liver and spleen.,","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":50411249664280,"sku":"ES3194","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ES3194-c-1.jpg?v=1751076954","url":"https:\/\/danabiosci.com\/products\/es3194","provider":"Dana Bioscience","version":"1.0","type":"link"}