{"product_id":"es2300","title":"ERK 8 rabbit pAb","description":"\u003cstrong\u003eApplications:\u003c\/strong\u003e WB;IHC;IF;ELISA\u003cbr\u003e\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human;Mouse\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Rabbit\u003cbr\u003e\u003cstrong\u003eDilution:\u003c\/strong\u003e Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/10000. Not yet tested in other applications.\u003cbr\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e The antiserum was produced against synthesized peptide derived from human MAPK15. AA range:141-190\u003cbr\u003e\u003cstrong\u003eStorage_stability:\u003c\/strong\u003e -20°C\/1 year\u003cbr\u003e\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal\u003cbr\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG\u003cbr\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1 mg\/ml\u003cbr\u003e\u003cstrong\u003eObserved_band(KD):\u003c\/strong\u003e 60kD\u003cbr\u003e\u003cstrong\u003eHuman_gene_id:\u003c\/strong\u003e 225689\u003cbr\u003e\u003cstrong\u003eHuman_swiss_prot_no:\u003c\/strong\u003e Q8TD08\u003cbr\u003e\u003cstrong\u003eSubcellular_location:\u003c\/strong\u003e Cytoplasm, cytoskeleton, cilium basal body . Cell junction, tight junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasmic vesicle, autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity). .\u003cbr\u003e\u003cstrong\u003eOther_name:\u003c\/strong\u003e MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser\/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK\/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney.,","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":50411190026520,"sku":"ES2300","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ES2300-c-1.jpg?v=1751073954","url":"https:\/\/danabiosci.com\/products\/es2300","provider":"Dana Bioscience","version":"1.0","type":"link"}