{"product_id":"es1425","title":"Rad17 (phospho Ser645) rabbit pAb","description":"\u003cstrong\u003eApplications:\u003c\/strong\u003e WB; ELISA;IHC\u003cbr\u003e\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human;Mouse\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Rabbit\u003cbr\u003e\u003cstrong\u003eDilution:\u003c\/strong\u003e WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000\u003cbr\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e The antiserum was produced against synthesized peptide derived from human RAD17 around the phosphorylation site of Ser645. AA range:621-670\u003cbr\u003e\u003cstrong\u003eStorage_stability:\u003c\/strong\u003e -20°C\/1 year\u003cbr\u003e\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal\u003cbr\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG\u003cbr\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1 mg\/ml\u003cbr\u003e\u003cstrong\u003eObserved_band(KD):\u003c\/strong\u003e 77kD\u003cbr\u003e\u003cstrong\u003eHuman_gene_id:\u003c\/strong\u003e 5884\u003cbr\u003e\u003cstrong\u003eHuman_swiss_prot_no:\u003c\/strong\u003e O75943\u003cbr\u003e\u003cstrong\u003eSubcellular_location:\u003c\/strong\u003e Nucleus . Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.\u003cbr\u003e\u003cstrong\u003eOther_name:\u003c\/strong\u003e RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C\/activator 1 homolog\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":50411104796952,"sku":"ES1425","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ES1425-c-1.jpg?v=1751070858","url":"https:\/\/danabiosci.com\/products\/es1425","provider":"Dana Bioscience","version":"1.0","type":"link"}