{"product_id":"es1353","title":"LIMK-2 (phospho Thr505) rabbit pAb","description":"\u003cstrong\u003eApplications:\u003c\/strong\u003e WB;IHC;IF;ELISA\u003cbr\u003e\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human;Mouse;Rat\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Rabbit\u003cbr\u003e\u003cstrong\u003eDilution:\u003c\/strong\u003e Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/10000. Not yet tested in other applications.\u003cbr\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Synthesized phospho-peptide around the phosphorylation site of human LIMK-2 (phospho Thr505)\u003cbr\u003e\u003cstrong\u003eStorage_stability:\u003c\/strong\u003e -20°C\/1 year\u003cbr\u003e\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal\u003cbr\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG\u003cbr\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1 mg\/ml\u003cbr\u003e\u003cstrong\u003eHuman_gene_id:\u003c\/strong\u003e 3985\u003cbr\u003e\u003cstrong\u003eHuman_swiss_prot_no:\u003c\/strong\u003e P53671\u003cbr\u003e\u003cstrong\u003eSubcellular_location:\u003c\/strong\u003e Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .; [Isoform LIMK2a]: Cytoplasm . Nucleus .; [Isoform LIMK2b]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus . Mainly present in the cytoplasm and is scarcely translocated to the nucleus. .\u003cbr\u003e\u003cstrong\u003eOther_name:\u003c\/strong\u003e LIMK2; LIM domain kinase 2; LIMK-2\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":50411093098776,"sku":"ES1353","price":250.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/danabiosci.com\/products\/es1353","provider":"Dana Bioscience","version":"1.0","type":"link"}