{"product_id":"es1078","title":"DDDDK-Tag(binds to flag sequnence) rabbit pAb","description":"\u003cstrong\u003eApplications:\u003c\/strong\u003e WB;ELISA;IP;IF\u003cbr\u003e\u003cstrong\u003eReactivity:\u003c\/strong\u003e Species independent\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Rabbit\u003cbr\u003e\u003cstrong\u003eDilution:\u003c\/strong\u003e Western Blot: 1\/1000 - 1\/3000. ELISA: 1:5000-20000. Not yet tested in other applications.\u003cbr\u003e\u003cstrong\u003eImmunogen:\u003c\/strong\u003e DDDDK synthetic peptide conjugated to KLH.\u003cbr\u003e\u003cstrong\u003eStorage_stability:\u003c\/strong\u003e -20°C\/1 year\u003cbr\u003e\u003cstrong\u003eClonality:\u003c\/strong\u003e Polyclonal\u003cbr\u003e\u003cstrong\u003eIsotype:\u003c\/strong\u003e IgG\u003cbr\u003e\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1 mg\/ml\u003cbr\u003e\u003cstrong\u003eOther_name:\u003c\/strong\u003e DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":50411067015448,"sku":"ES1078","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ES1078-c-1.jpg?v=1751069966","url":"https:\/\/danabiosci.com\/products\/es1078","provider":"Dana Bioscience","version":"1.0","type":"link"}