{"product_id":"ept283","title":"Biotinylated Human Mesothelin (C-6His-Avi)","description":"\u003cstrong\u003eDescription:\u003c\/strong\u003e Biotinylated Recombinant Human Mesothelin  is produced by our Mammalian expression system and the target gene encoding Glu296-Ser598 is expressed with a 6His, Avi tag at the C-terminus.\u003cbr\u003e\u003cstrong\u003eAccession:\u003c\/strong\u003e AAH09272.1\u003cbr\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 36.7 KDa\u003cbr\u003e\u003cstrong\u003eApparent molecular weight:\u003c\/strong\u003e 38-60 KDa, reducing conditions\u003cbr\u003e\u003cstrong\u003eOther names:\u003c\/strong\u003e Megakaryocyte potentiating factor; mesothelin; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein; CAK1; MPF; MSLN; SMR; CAK1; CAK1 antigen\u003cbr\u003e\u003cstrong\u003eStorage condition:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by reducing SDS-PAGE.\u003cbr\u003e\u003cstrong\u003eEndotoxin:\u003c\/strong\u003e Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003cbr\u003e\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Loaded Anti-Human Mesothelin  mAb-Fc(Cat#NC078) on Protein A Biosensor, can bind Biotinylated Human Mesothelin-His-Avi(Cat#CY05) with an affinity constant of 10\u003csup\u003e-3\u003c\/sup\u003e nM as determined in BLI assay.\u003cbr\u003e\u003cstrong\u003eRedissolve:\u003c\/strong\u003e Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003eDelivery condition:\u003c\/strong\u003e The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa (pleura, pericardium, and peritoneum) and epithelial cells of the trachea, tonsils, fallopian tube, and kidneys. Mesothelin plays an important role in cell survival, proliferation, migration, invasion, tumor progression, and resistance to chemotherapy. The overexpression of mesothelin can activate NF-κB and signal transducer and activator of transcription 3 (Stat3), inhibit apoptotic signaling and TNF-α-induced apoptosis, and accelerate the G1–S transition. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy.","brand":"ELK Biotechnology","offers":[{"title":"100ug","offer_id":50413950140696,"sku":"EPT283","price":1439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_b064aae4-b6f3-4263-9237-13f828d6a645.jpg?v=1751164310","url":"https:\/\/danabiosci.com\/products\/ept283","provider":"Dana Bioscience","version":"1.0","type":"link"}