{"product_id":"ept169","title":"Recombinant Human APRIL (N-Flag-His)","description":"\u003cstrong\u003eDescription:\u003c\/strong\u003e Recombinant Human A proliferation-inducing Ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus.\u003cbr\u003e\u003cstrong\u003eAccession:\u003c\/strong\u003e O75888\u003cbr\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 50 KDa\u003cbr\u003e\u003cstrong\u003eApparent molecular weight:\u003c\/strong\u003e 60 KDa, reducing conditions\u003cbr\u003e\u003cstrong\u003eOther names:\u003c\/strong\u003e Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13\u003cbr\u003e\u003cstrong\u003eStorage condition:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by reducing SDS-PAGE.\u003cbr\u003e\u003cstrong\u003eEndotoxin:\u003c\/strong\u003e Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003cbr\u003e\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Immobilized Human APRIL -Flag-His(Cat#CU89) at 1μg\/ml (100 μl\/well) can bind Cynomolgus BCMA-Fc(Cat#CP59).The ED50 of  Cynomolgus BCMA-Fc(Cat#CP59) is 4.23 ng\/ml.\u003cbr\u003e\u003cstrong\u003eRedissolve:\u003c\/strong\u003e Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003eDelivery condition:\u003c\/strong\u003e The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses. APRIL levels are elevated in the serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratin neutrophils.","brand":"ELK Biotechnology","offers":[{"title":"10ug","offer_id":50413838958872,"sku":"EPT169","price":199.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_731a9f58-58f7-495d-b5e3-b40b62c85f15.jpg?v=1751163959","url":"https:\/\/danabiosci.com\/products\/ept169","provider":"Dana Bioscience","version":"1.0","type":"link"}