{"product_id":"ept114","title":"Recombinant 2019-nCoV Papain-Like Protease","description":"\u003cstrong\u003eDescription:\u003c\/strong\u003e Recombinant 2019-nCoV Papain-like Protease is produced by our E.coli expression system and the target gene encoding Glu1564-Lys1878 is expressed.\u003cbr\u003e\u003cstrong\u003eAccession:\u003c\/strong\u003e QHD43415.1\u003cbr\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 35.8 KDa\u003cbr\u003e\u003cstrong\u003eApparent molecular weight:\u003c\/strong\u003e 34 KDa, reducing conditions\u003cbr\u003e\u003cstrong\u003eOther names:\u003c\/strong\u003e Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a\u003cbr\u003e\u003cstrong\u003eStorage condition:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by reducing SDS-PAGE.\u003cbr\u003e\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol\/min\/μg.\u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at ≤-70°C, stable for 6 months after receipt.\nStore at ≤-70°C, stable for 3 months under sterile conditions after opening. \nPlease minimize freeze-thaw cycles.      \u003cbr\u003e\u003cstrong\u003eDelivery condition:\u003c\/strong\u003e The product is shipped on dry ice\/polar packs.\nUpon receipt, store it immediately at the temperature listed below.\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.","brand":"ELK Biotechnology","offers":[{"title":"50ug","offer_id":50413836239128,"sku":"EPT114","price":839.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_d8945e66-d4c4-4f22-96a4-d51c0f053726.jpg?v=1751163781","url":"https:\/\/danabiosci.com\/products\/ept114","provider":"Dana Bioscience","version":"1.0","type":"link"}