{"product_id":"ept111","title":"Recombinant Mouse SHH (C25II)","description":"\u003cstrong\u003eDescription:\u003c\/strong\u003e Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198(Cys25Ile-Ile) is expressed.\u003cbr\u003e\u003cstrong\u003eAccession:\u003c\/strong\u003e Q62226\u003cbr\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 19.8 KDa\u003cbr\u003e\u003cstrong\u003eApparent molecular weight:\u003c\/strong\u003e 18-20  KDa, reducing conditions\u003cbr\u003e\u003cstrong\u003eOther names:\u003c\/strong\u003e Sonic Hedgehog Protein; SHH; HHG-1; SHH\u003cbr\u003e\u003cstrong\u003eStorage condition:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by reducing SDS-PAGE.\u003cbr\u003e\u003cstrong\u003eEndotoxin:\u003c\/strong\u003e Less than 0.001 ng\/µg (0.01 EU\/µg) as determined by LAL test.\u003cbr\u003e\u003cstrong\u003eRedissolve:\u003c\/strong\u003e Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003eDelivery condition:\u003c\/strong\u003e The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N­terminal fragment (Shh­N), and a catalytic­processing,glycosylated, 34 kDa, 239 aa C­terminal fragment. The 20 kDa Shh­N fragment is the core of the active hedgehog molecule. Mouse Shh­N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh­N, respectively.","brand":"ELK Biotechnology","offers":[{"title":"50ug","offer_id":50413836140824,"sku":"EPT111","price":589.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_d97c1542-e4fc-44b9-8661-75021ab67907.jpg?v=1751163773","url":"https:\/\/danabiosci.com\/products\/ept111","provider":"Dana Bioscience","version":"1.0","type":"link"}