{"product_id":"ept106","title":"Recombinant Human PD-1 (C-Fc)","description":"\u003cstrong\u003eDescription:\u003c\/strong\u003e Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a Fc tag at the C-terminus.\u003cbr\u003e\u003cstrong\u003eAccession:\u003c\/strong\u003e Q15116\u003cbr\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 43.6 KDa\u003cbr\u003e\u003cstrong\u003eApparent molecular weight:\u003c\/strong\u003e 60-70 KDa, reducing conditions\u003cbr\u003e\u003cstrong\u003eOther names:\u003c\/strong\u003e Programmed cell death protein 1; PDCD1; PD-1; hPD-1; CD279\u003cbr\u003e\u003cstrong\u003eStorage condition:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 95% as determined by reducing SDS-PAGE.\u003cbr\u003e\u003cstrong\u003eEndotoxin:\u003c\/strong\u003e Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003cbr\u003e\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Immobilized Human PD-1-Fc (Cat#CM81) at 2μg\/ml (100 μl\/well) can bind Anti-Human PD-1 mAb(Cat#GMP-A085).  The ED50 of Human Anti-Human PD-1 mAb(Cat#GMP-A085) is 48.76 ng\/ml.\u003cbr\u003e\u003cstrong\u003eRedissolve:\u003c\/strong\u003e Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003eDelivery condition:\u003c\/strong\u003e The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28\/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K\/AKT pathway. In addition, coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.","brand":"ELK Biotechnology","offers":[{"title":"50ug","offer_id":50413835944216,"sku":"EPT106","price":339.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_037024b2-2745-45db-9d1b-93418fe8533f.jpg?v=1751163759","url":"https:\/\/danabiosci.com\/products\/ept106","provider":"Dana Bioscience","version":"1.0","type":"link"}