{"product_id":"ept080","title":"Recombinant 2019-nCoV Helicase (N-6His-MBP)","description":"\u003cstrong\u003eDescription:\u003c\/strong\u003e Recombinant 2019-nCoV Helicase is produced by our E.coli expression system and the target gene encoding Ala5325-Gln5925 is expressed with a 6His, MBP tag at the N-terminus.\u003cbr\u003e\u003cstrong\u003eAccession:\u003c\/strong\u003e P0DTD1\u003cbr\u003e\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 112.8 KDa\u003cbr\u003e\u003cstrong\u003eApparent molecular weight:\u003c\/strong\u003e 120 KDa, reducing conditions\u003cbr\u003e\u003cstrong\u003eOther names:\u003c\/strong\u003e SARS-CoV 2 Helicase; SARS-CoV 2 nsp13 \u003cbr\u003e\u003cstrong\u003eStorage condition:\u003c\/strong\u003e Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003cbr\u003e\u003cstrong\u003ePurity:\u003c\/strong\u003e Greater than 80% as determined by reducing SDS-PAGE.\u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at ≤-70°C, stable for 6 months after receipt.\nStore at ≤-70°C, stable for 3 months under sterile conditions after opening. \nPlease minimize freeze-thaw cycles.      \u003cbr\u003e\u003cstrong\u003eDelivery condition:\u003c\/strong\u003e The product is shipped on dry ice\/polar packs.\nUpon receipt, store it immediately at the temperature listed below.\u003cbr\u003e\u003cstrong\u003eBackground:\u003c\/strong\u003e The non—structural protein 13 (nsp13) of SARS—CoV 2 is a helicase that separates double—stranded RNA or DNA with a 5'—3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.","brand":"ELK Biotechnology","offers":[{"title":"50ug","offer_id":50413834731800,"sku":"EPT080","price":699.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_0065fde8-26c5-48f3-ab50-ebc45ec07529.jpg?v=1751163681","url":"https:\/\/danabiosci.com\/products\/ept080","provider":"Dana Bioscience","version":"1.0","type":"link"}