{"product_id":"elk9922","title":"Pig HSPA5(Heat Shock 70kDa Protein 5) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e 78 kDa glucose-regulated protein; GRP-78; Binding-immunoglobulin protein; BiP; HSP70 family protein 5;  Heat shock protein family A member 5; Immunoglobulin heavy chain-binding protein; Endoplasmic reticulum chaperone BiP; BIP\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.067 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 10 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.16-10 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;Tumor immunity;Infection immunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HSPA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HSPA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HSPA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HSPA5 in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410771743000,"sku":"ELK9922","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_765e3f3e-4c02-4465-99c6-847d093ce6c3.jpg?v=1751055981","url":"https:\/\/danabiosci.com\/products\/elk9922","provider":"Dana Bioscience","version":"1.0","type":"link"}