{"product_id":"elk9828","title":"Human ABL1(Tyrosine-protein kinase ABL1) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 17 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 4000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 62.5-4000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal Transduction; Cancer; Epigenetics and Nuclear Signaling; Neuroscience\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABL1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P00519","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410767679768,"sku":"ELK9828","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_b3a99169-ce5b-451a-a8d8-4ea8ace715dd.jpg?v=1751055705","url":"https:\/\/danabiosci.com\/products\/elk9828","provider":"Dana Bioscience","version":"1.0","type":"link"}