{"product_id":"elk9777","title":"Rat Phospho-Akt (S473) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e p-AKT\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 6.4 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 1000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 15.63-1000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e tissue homogenates, cell lysates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Enzyme \u0026amp; Kinase;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Phospho-Akt. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Phospho-Akt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Phospho-Akt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Phospho-Akt in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410765091096,"sku":"ELK9777","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_2d1035db-a6a9-47c3-9c78-9346752c8ba3.jpg?v=1751055547","url":"https:\/\/danabiosci.com\/products\/elk9777","provider":"Dana Bioscience","version":"1.0","type":"link"}