{"product_id":"elk9587","title":"Human N-MID-OT(N-MIDOsteocalcin) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e N-MID-OT\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.39 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 40 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.63-40 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human N-MID-OT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N-MID-OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human N-MID-OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human N-MID-OT in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410744152344,"sku":"ELK9587","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_2fdb9010-e73e-4004-91f3-abd7a77b1399.jpg?v=1751055014","url":"https:\/\/danabiosci.com\/products\/elk9587","provider":"Dana Bioscience","version":"1.0","type":"link"}