{"product_id":"elk9408","title":"Hamster IL10(Interleukin 10) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e IL-10;CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 2.64 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 400 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 6.25-400 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Cytokine;Infection immunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster IL10 in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410717741336,"sku":"ELK9408","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_50adad4d-701f-4ded-9a7a-9ba73bb7543a.jpg?v=1751054550","url":"https:\/\/danabiosci.com\/products\/elk9408","provider":"Dana Bioscience","version":"1.0","type":"link"}