{"product_id":"elk9082","title":"Chicken TLR4(Toll Like Receptor 4) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e CD284; TOLL; HToll\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.069 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 10 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.16-10 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;Enzyme \u0026amp; Kinase;Tumor immunity;Infection immunity;Pulmonology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken TLR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken TLR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken TLR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken TLR4 in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410670457112,"sku":"ELK9082","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_c8613f6e-2598-42a9-8ccc-8d0a830c99c3.jpg?v=1751053626","url":"https:\/\/danabiosci.com\/products\/elk9082","provider":"Dana Bioscience","version":"1.0","type":"link"}