{"product_id":"elk9031","title":"Estrogen ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e E ; Estrogen\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 4.01 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 1000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 15.625-1000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Reproductive science;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrogen protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410665574680,"sku":"ELK9031","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_e345d5f4-f5bf-4c26-80b7-ad48944d46dd.jpg?v=1751053490","url":"https:\/\/danabiosci.com\/products\/elk9031","provider":"Dana Bioscience","version":"1.0","type":"link"}