{"product_id":"elk8374","title":"Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 1.1 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 150 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 2.35-150 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma and other biological fluids.\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410623664408,"sku":"ELK8374","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_7bc066c3-a420-4556-8d0b-3363c070961c.jpg?v=1751051798","url":"https:\/\/danabiosci.com\/products\/elk8374","provider":"Dana Bioscience","version":"1.0","type":"link"}