{"product_id":"elk8273","title":"S1P(Sphingosine-1-Phosphate) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Sphingosine-1-Phosphate\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 4.48 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 1000 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 15.63-1000 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Ovarian dysfunction\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S1P protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1P in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410620191000,"sku":"ELK8273","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_bebc41dc-be9b-4850-aff0-9851b1f7a8dc.jpg?v=1751051658","url":"https:\/\/danabiosci.com\/products\/elk8273","provider":"Dana Bioscience","version":"1.0","type":"link"}