{"product_id":"elk8250","title":"PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e PtdIns(3,4,5)P3\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 2.84 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 500 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 7.82-500 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410619273496,"sku":"ELK8250","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_7907521d-617d-4f1d-b864-8eb9767e3e34.jpg?v=1751051595","url":"https:\/\/danabiosci.com\/products\/elk8250","provider":"Dana Bioscience","version":"1.0","type":"link"}