{"product_id":"elk8171","title":"SPH(Sphingomyelin) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e SM\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 1.82 µg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 400 µg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 6.25-400 µg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;Neuro science;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SPH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPH in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410613997848,"sku":"ELK8171","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_5d34d698-37c0-4902-837e-20464ebb156e.jpg?v=1751051403","url":"https:\/\/danabiosci.com\/products\/elk8171","provider":"Dana Bioscience","version":"1.0","type":"link"}