{"product_id":"elk8028","title":"Cattle FETUB(Fetuin B) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e FETU-B; 16G2; Gugu; IRL685; Fetuin-like protein IRL685\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 11.9 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 2000 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 31.25-2000 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma and other biological fluids.\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;Infection immunity;Endocrinology;Bone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle FETUB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle FETUB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle FETUB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle FETUB in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q58D62","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410599842072,"sku":"ELK8028","price":688.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_8901f126-a5d1-4192-a554-09444bebc111.jpg?v=1751051009","url":"https:\/\/danabiosci.com\/products\/elk8028","provider":"Dana Bioscience","version":"1.0","type":"link"}