{"product_id":"elk8020","title":"NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e NAADP\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 51 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 10000 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 156.25-10000 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;Enzyme \u0026amp; Kinase;Metabolic pathway;Tumor immunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410599612696,"sku":"ELK8020","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_c98ab007-ce7c-433e-aa5a-97351c42a7a0.jpg?v=1751050989","url":"https:\/\/danabiosci.com\/products\/elk8020","provider":"Dana Bioscience","version":"1.0","type":"link"}