{"product_id":"elk7054","title":"Guinea pig VIP(Vasoactive Intestinal Peptide) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 2.42 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 500 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 7.82-500 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma and other biological fluids.\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Endocrinology;Gastroenterology;Hormone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig VIP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P04566","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410504487192,"sku":"ELK7054","price":688.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_83134601-257a-4696-8621-2203451cecf2.jpg?v=1751048321","url":"https:\/\/danabiosci.com\/products\/elk7054","provider":"Dana Bioscience","version":"1.0","type":"link"}