{"product_id":"elk6274","title":"Rat EDN2(Endothelin 2) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e ET2; PPET2; Preproendothelin-2\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 2.66 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 500 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 7.82-500 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Cardiovascular biology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat EDN2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P23943","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410063921432,"sku":"ELK6274","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_f9f2ada2-d33d-4713-89d7-7dc39b5b4652.jpg?v=1751016231","url":"https:\/\/danabiosci.com\/products\/elk6274","provider":"Dana Bioscience","version":"1.0","type":"link"}